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Sampling Methods

TECH GUIDE

Fish

Monitoring the health of fish in streams, rivers, lakes, and estuaries is important for several reasons. Fish are an excellent indicator species since they are at the top of the food web and tend to integrate effects observed in lower trophic levels. In addition, fish have a high economic value and impacts to fish populations can significantly influence the food chain. Effects on fish can be measured at the cellular level, the individual level (i.e. to obtain tissue metals concentrations, health and condition), the population level (i.e. changes in growth and fertility), and the community level (i.e. ecological effects such as changes in species composition, relative abundance and distribution). The decision to measure effects at the cellular, individual, population, or community level will depend upon study objectives.

Fish are sampled during baseline monitoring programs to document species composition, population size, and document population characteristics (i.e. length-weight relationships, age-structure, sex ratio, fertility, growth). Sampling fish tissue to determine metals concentrations is also common. Information on population characteristics and metals concentrations is collected before, during, and after closure of the mine in order to track environmental impacts (MEND, 2001).

Stream and River Sampling

Measuring fish population abundance in streams is usually accomplished by removal methods that involve temporarily removing the majority of fish in a small reach. The most common methods are beach seining (where possible) and electro-fishing. Beach seining is accomplished by setting nets in a reach (i.e. 100 m) to prevent immigration and emigration of fish and conducting sweeps of the reach to enumerate all fish. Fish are returned to the stream once sampling has been completed and changes in abundance over time are monitored. Electro-fishing can be used in conjunction with beach seining. Electro-fishing is generally conducted by boat and can access difficult locations (i.e. narrow streams and rivers) that are difficult to access using beach seining alone. Individual fish can be acquired from each of the capture methods to measure tissue metal concentrations (MEND, 2001).

Lake and Estuary Sampling

The abundance and condition of fish populations in lakes and estuaries are usually measured using nets (e.g. gill nets and trap nets) and by angling. Studies conducted in large waterbodies are normally focused at the population or community level, and not at the individual level. To determine possible effects on fish populations or communities, information on species composition, abundance, and distribution of fish is required. This requires that many fish must be captured from a relatively large area using quantitative, non-destructive techniques. For example, changes in relative abundance of lake, estuarine and marine fish populations are generally estimated using catch-per-unit-effort (CPUE) statistics (i.e. number of fish captured per 100 m of net per hour). The same areas are fished repeatedly over time to determine changes in the number of fish captured per unit time. Changes in CPUE are indicative of changes in fish species composition and relative abundance at the community level. In addition, changes at the population level such as growth, fertility, condition factor, and age-frequency distribution can be used to determine effects. Such studies are normally conducted over long time periods and require specialized knowledge. Fish for tissue analyses can be collected using any of the methods used for population sampling. Minnow traps target smaller fish that are not easily sampled with nets and may be appropriate for use in some lakes (MEND, 2001).

 

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